We revise the frogs of the genus Phrynoglossus from Indochina based on data of external morphology, bioacoustics, and molecular genetics. The results of this integrative study provide evidence for the recognition of three distinct species, one of which we describe as new. Phrynoglossus martensii has a vast geographic distribution from central and southern Thailand across southern China to Vietnam, Laos, and Cambodia. Phrynoglossus myanhessei sp. nov. is distributed in central Myanmar whereas Phrynoglossus magnapustulosus is restricted to the Khorat Plateau, Thailand. These three species occur in allopatry and differ in their mating calls, external morphology, and genetic distances of the 16S gene of 3.8–5.9%. Finally, we discuss and provide evolutionary evidence for the recognition of Phrynoglossus as a genus distinct from Occidozyga. Members of both genera form reciprocal monophyletic groups in our analyses of mtDNA data (16S) and are well differentiated from each other in morphology and ecology. Furthermore, they differ in the amplexus mode with Phrynoglossus having an inguinal amplexus whereas it is axillary in Occidozyga. We further provide a de novo draft genome of the holotype based on short-read sequencing technology to coverage of 25-fold. This resource will permanently link the genetic characterization of the species to the name-bearing type specimen.