Herbarium material collected in 2009–2013 in Cambodia and Laos provides 240 new localities for 156 orchid species (from 73 genera). Among them, 13 and 45 species respectively are new for the flora of each country. One species (Bulbophyllum konstantinovii) discovered in Cambodia is described as new for science. Eight genera (Acanthephippium, Didymoplexiopsis, Eclecticus, Herpysma, Hetaeria, Lecanorchis, Neuwiedia, and Trichosma) were found in Laos at first.