Two new species of Annonaceae, Monoon namkadingense and Neo-uvaria laosensis, from Nam Kading National Protected Area, Bolikhamxay Province, Central Laos, are described and illustrated. DNA barcodes of rbcL and matK, vernacular name and primary conservation assessment are also provided. Both species are considered to be endemic to this area and categorized as Endangered (EN). The distribution of Neo-uvaria laosensis represents northern limit of distribution range for the genus.